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KMID : 0043319980210030315
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Archives of Pharmacal Research
1998 Volume.21 No. 3 p.315 ~ p.319
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The Involvement of Protein Kinase C and Tyrosine Kinase in Vanadate-induced Contraction
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Sim Sang-Soo
Kim Chang-Jong
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Abstract
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Gastric smooth muscle of cats was used to investigate the involvement of protein kinase in vanadate-induced contraction. Vanadate caused a contraction of cat gastric smooth muscle in a dose-dependent manner. Vanadate-induced contraction was totally inhibited by 2 mM EGTA and 1.5 mM and significantly inhibited by M verapamil and M nifedipine, suggesting that vanadate-induced contraction is dependent on the extracellular concentration, and the influx of extracellular was mediated through voltage-dependent channel. Both protein kinase C inhibitor and tyrosine kinase inhibitor significantly inhibited the vanadate-induced contraction and the combined inhibitory effect of two protein kinase inhibitors was greater than that of each one. But calmodulin antagonists did not have any influence on the vanadate-induced contraction. On the other hand, both forskolin (M) and sodium nitroprusside (M) significantly inhibited vanadate-induced contraction. Therefore, these results suggest that both protein kinase C and tyrosino kinase are involved in the vanadate-induced contraction which required the influx of extracellular in cat gastric smooth muscle, and that the contractile mechanism of vanadate may be different from that of agonist binding to its specific receptor.
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KEYWORD
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Calcium channel antagonist, Protein Kinase C, Smooth muscle contraction, Tyrosine kinase
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